2 3 bis Search Results


bis t 23  (TargetMol)
93
TargetMol bis t 23
Cytoskeleton stabilization reduces tubular cell apoptosis in UUO mouse models. ( A ) Representative TUNEL staining images of kidney sections from sham, UUO, and <t>UUO</t> <t>+</t> <t>Bis-T-23</t> groups, highlighting apoptotic cells. Scale bars, 50 μm. ( B ) Quantification of TUNEL-positive cells per high-power field (HPF). ( C ) Western blot analysis of apoptosis-related proteins BAX, Bcl-2, caspase-3, and cleaved caspase-3 in kidney tissues from different groups. ( D ) Quantitative analysis of BAX/Bcl-2 and cleaved caspase-3/caspase-3 protein expression ratios. ( E ) Immunohistochemical staining of BAX and Bcl-2 in kidney tissues, showing changes in pro-apoptotic and anti-apoptotic protein expression. Scale bars, 50 μm. Data are presented as mean±SD. **P < 0.01 vs sham group; *P < 0.05 vs UUO group.
Bis T 23, supplied by TargetMol, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/2+3+bis/Bis-T-23/pmc12417705-4-23-24
Average 93 stars, based on 1 article reviews
bis t 23 - by Bioz Stars, 2026-10
93/100 stars
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verhandlungen der sechzehnten versammlung deutscher philologen, schulmänner und orientalisten in stuttgart vom 23. bis 26  (Bachofen AG)
90
Bachofen AG verhandlungen der sechzehnten versammlung deutscher philologen, schulmänner und orientalisten in stuttgart vom 23. bis 26
Cytoskeleton stabilization reduces tubular cell apoptosis in UUO mouse models. ( A ) Representative TUNEL staining images of kidney sections from sham, UUO, and <t>UUO</t> <t>+</t> <t>Bis-T-23</t> groups, highlighting apoptotic cells. Scale bars, 50 μm. ( B ) Quantification of TUNEL-positive cells per high-power field (HPF). ( C ) Western blot analysis of apoptosis-related proteins BAX, Bcl-2, caspase-3, and cleaved caspase-3 in kidney tissues from different groups. ( D ) Quantitative analysis of BAX/Bcl-2 and cleaved caspase-3/caspase-3 protein expression ratios. ( E ) Immunohistochemical staining of BAX and Bcl-2 in kidney tissues, showing changes in pro-apoptotic and anti-apoptotic protein expression. Scale bars, 50 μm. Data are presented as mean±SD. **P < 0.01 vs sham group; *P < 0.05 vs UUO group.
Verhandlungen Der Sechzehnten Versammlung Deutscher Philologen, Schulmänner Und Orientalisten In Stuttgart Vom 23. Bis 26, supplied by Bachofen AG, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/2+3+bis/verhandlungen+der+sechzehnten+versammlung+deutscher+philologen++schulm%C3%A4nner+und+orientalisten+in+stuttgart+vom+23++bis+26/10__1515_slash_9783110233834__153-193-5-0
Average 90 stars, based on 1 article reviews
verhandlungen der sechzehnten versammlung deutscher philologen, schulmänner und orientalisten in stuttgart vom 23. bis 26 - by Bioz Stars, 2026-10
90/100 stars
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1 23-bis(maleimido)heptaethyleneglycol  (Biosynth Carbosynth)
N/A
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Image Search Results


Cytoskeleton stabilization reduces tubular cell apoptosis in UUO mouse models. ( A ) Representative TUNEL staining images of kidney sections from sham, UUO, and UUO + Bis-T-23 groups, highlighting apoptotic cells. Scale bars, 50 μm. ( B ) Quantification of TUNEL-positive cells per high-power field (HPF). ( C ) Western blot analysis of apoptosis-related proteins BAX, Bcl-2, caspase-3, and cleaved caspase-3 in kidney tissues from different groups. ( D ) Quantitative analysis of BAX/Bcl-2 and cleaved caspase-3/caspase-3 protein expression ratios. ( E ) Immunohistochemical staining of BAX and Bcl-2 in kidney tissues, showing changes in pro-apoptotic and anti-apoptotic protein expression. Scale bars, 50 μm. Data are presented as mean±SD. **P < 0.01 vs sham group; *P < 0.05 vs UUO group.

Journal: Drug Design, Development and Therapy

Article Title: Cytoskeleton Rearrangement Decreases Mitochondrial Fatty Acid Oxidation in Renal Tubular Epithelial Cells Contributing to Renal Fibrogenesis

doi: 10.2147/DDDT.S520262

Figure Lengend Snippet: Cytoskeleton stabilization reduces tubular cell apoptosis in UUO mouse models. ( A ) Representative TUNEL staining images of kidney sections from sham, UUO, and UUO + Bis-T-23 groups, highlighting apoptotic cells. Scale bars, 50 μm. ( B ) Quantification of TUNEL-positive cells per high-power field (HPF). ( C ) Western blot analysis of apoptosis-related proteins BAX, Bcl-2, caspase-3, and cleaved caspase-3 in kidney tissues from different groups. ( D ) Quantitative analysis of BAX/Bcl-2 and cleaved caspase-3/caspase-3 protein expression ratios. ( E ) Immunohistochemical staining of BAX and Bcl-2 in kidney tissues, showing changes in pro-apoptotic and anti-apoptotic protein expression. Scale bars, 50 μm. Data are presented as mean±SD. **P < 0.01 vs sham group; *P < 0.05 vs UUO group.

Article Snippet: TGF-β1-treated primary tubular epithelial cells (PTECs) and UUO mice served as renal interstitial fibrosis (RIF) models, with or without pharmacological cytoskeleton stabilization by Bis-T-23 (TargetMol, T30479 ; 20 mg/kg/day intraperitoneally in vivo; 5 μM in vitro).

Techniques: TUNEL Assay, Staining, Western Blot, Expressing, Immunohistochemical staining

Cytoskeleton stabilization protects tubular epithelial cells from TGF-β1-induced EMT, apoptosis and metabolic dysfunction. ( A ) Immunofluorescence staining of cytokeratin-18 (red) confirming the epithelial origin and purity of primary tubular epithelial cells (PTECs) used for in vitro experiments, validating successful isolation of renal tubular epithelial cells. Nuclei were stained with DAPI (blue). Scale bar, 20 μm. ( B ) Cell viability of primary renal tubular epithelial cells treated with different concentrations of Bis-T-23. ( C ) Cell viability of primary renal tubular epithelial cells pre-treated with different concentrations of Bis-T-23, followed by TGF-β1 induction. ( D ) Morphological changes in tubular epithelial cells treated with normal media (N), Bis-T-23, TGF-β1, or TGF-β1+Bis-T-23. ( E ) Immunofluorescence staining of E-cadherin (red) and nuclei (DAPI, blue) to evaluate EMT induction. Scale bar, 20 μm. ( F ) Western blot analysis of fibrosis-related proteins (fibronectin, collagen I) and EMT markers (E-cadherin, α-SMA) in different treatment groups, with quantitative protein levels normalized to α-tubulin. ( G ) Western blot analysis of apoptosis-related proteins BAX, Bcl-2, caspase-3, and cleaved caspase-3 in different treatment groups. Quantitative analysis of BAX/Bcl-2 and cleaved caspase-3/caspase-3 protein expression ratios. ( H ) Western blot analysis of key fatty acid oxidation (FAO) enzymes and mitochondrial electron transport chain (ETC) complex proteins, including PPARα, ACOX1, CPT1A, and mitochondrial complexes I–V in different treatment groups, with quantitative protein levels normalized to β-actin. Data are presented as mean±SD.**P < 0.01 vs N group; *P < 0.05 vs TGF-β1 group.

Journal: Drug Design, Development and Therapy

Article Title: Cytoskeleton Rearrangement Decreases Mitochondrial Fatty Acid Oxidation in Renal Tubular Epithelial Cells Contributing to Renal Fibrogenesis

doi: 10.2147/DDDT.S520262

Figure Lengend Snippet: Cytoskeleton stabilization protects tubular epithelial cells from TGF-β1-induced EMT, apoptosis and metabolic dysfunction. ( A ) Immunofluorescence staining of cytokeratin-18 (red) confirming the epithelial origin and purity of primary tubular epithelial cells (PTECs) used for in vitro experiments, validating successful isolation of renal tubular epithelial cells. Nuclei were stained with DAPI (blue). Scale bar, 20 μm. ( B ) Cell viability of primary renal tubular epithelial cells treated with different concentrations of Bis-T-23. ( C ) Cell viability of primary renal tubular epithelial cells pre-treated with different concentrations of Bis-T-23, followed by TGF-β1 induction. ( D ) Morphological changes in tubular epithelial cells treated with normal media (N), Bis-T-23, TGF-β1, or TGF-β1+Bis-T-23. ( E ) Immunofluorescence staining of E-cadherin (red) and nuclei (DAPI, blue) to evaluate EMT induction. Scale bar, 20 μm. ( F ) Western blot analysis of fibrosis-related proteins (fibronectin, collagen I) and EMT markers (E-cadherin, α-SMA) in different treatment groups, with quantitative protein levels normalized to α-tubulin. ( G ) Western blot analysis of apoptosis-related proteins BAX, Bcl-2, caspase-3, and cleaved caspase-3 in different treatment groups. Quantitative analysis of BAX/Bcl-2 and cleaved caspase-3/caspase-3 protein expression ratios. ( H ) Western blot analysis of key fatty acid oxidation (FAO) enzymes and mitochondrial electron transport chain (ETC) complex proteins, including PPARα, ACOX1, CPT1A, and mitochondrial complexes I–V in different treatment groups, with quantitative protein levels normalized to β-actin. Data are presented as mean±SD.**P < 0.01 vs N group; *P < 0.05 vs TGF-β1 group.

Article Snippet: TGF-β1-treated primary tubular epithelial cells (PTECs) and UUO mice served as renal interstitial fibrosis (RIF) models, with or without pharmacological cytoskeleton stabilization by Bis-T-23 (TargetMol, T30479 ; 20 mg/kg/day intraperitoneally in vivo; 5 μM in vitro).

Techniques: Immunofluorescence, Staining, In Vitro, Isolation, Western Blot, Expressing